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ez-magna rip rna-binding protein immunoprecipitation kit  (Millipore)

 
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    Structured Review

    Millipore ez-magna rip rna-binding protein immunoprecipitation kit
    miR-516b-5p is a miRNA binding to hsa_circ_0072107. (A) Venn diagram showed that two miRNAs may bind to hsa_circ_0072107. (B) The binding site information of miR-516b-5p and miR-510-5p on hsa_circ_0072107. (C) Reverse transcription-quantitative PCR validation of transfection efficiency in cells transfected with miR-NC, miR-516b-5p mimics and inhibitors. (D) hsa_circ_0072107, miR-516b-5p and miR-510-5p levels in RNA enriched by circ_0072107 probe or scramble probe during biotinylated probe pull-down assay. (E) hsa_circ_0072107 level in miRNA complexes enriched by anti-AGO or IgG during anti-AGO2 <t>RIP</t> assay. AC16 cells transfected with miR-516b-5p mimic or miR-NC were used. (F) The effect of miR-516b-5p mimic or miR-NC on the relative luciferase activity of luciferase reporter with linear circ_0072107 or linear mutcirc_0072107 as the artificial 3′ UTR of Renilla , respectively. *P<0.05, **P<0.01, n=3. miRNA, microRNA; AGO2, argonaute 2; RIP, RNA <t>immunoprecipitation;</t> NC, negative control.
    Ez Magna Rip Rna Binding Protein Immunoprecipitation Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez-magna rip rna-binding protein immunoprecipitation kit/product/Millipore
    Average 90 stars, based on 1 article reviews
    ez-magna rip rna-binding protein immunoprecipitation kit - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "circRNA hsa_circ_0072107 aggravates myocardial hypertrophy via its function as a competitive endogenous RNA of miR-516b-5p"

    Article Title: circRNA hsa_circ_0072107 aggravates myocardial hypertrophy via its function as a competitive endogenous RNA of miR-516b-5p

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2025.13597

    miR-516b-5p is a miRNA binding to hsa_circ_0072107. (A) Venn diagram showed that two miRNAs may bind to hsa_circ_0072107. (B) The binding site information of miR-516b-5p and miR-510-5p on hsa_circ_0072107. (C) Reverse transcription-quantitative PCR validation of transfection efficiency in cells transfected with miR-NC, miR-516b-5p mimics and inhibitors. (D) hsa_circ_0072107, miR-516b-5p and miR-510-5p levels in RNA enriched by circ_0072107 probe or scramble probe during biotinylated probe pull-down assay. (E) hsa_circ_0072107 level in miRNA complexes enriched by anti-AGO or IgG during anti-AGO2 RIP assay. AC16 cells transfected with miR-516b-5p mimic or miR-NC were used. (F) The effect of miR-516b-5p mimic or miR-NC on the relative luciferase activity of luciferase reporter with linear circ_0072107 or linear mutcirc_0072107 as the artificial 3′ UTR of Renilla , respectively. *P<0.05, **P<0.01, n=3. miRNA, microRNA; AGO2, argonaute 2; RIP, RNA immunoprecipitation; NC, negative control.
    Figure Legend Snippet: miR-516b-5p is a miRNA binding to hsa_circ_0072107. (A) Venn diagram showed that two miRNAs may bind to hsa_circ_0072107. (B) The binding site information of miR-516b-5p and miR-510-5p on hsa_circ_0072107. (C) Reverse transcription-quantitative PCR validation of transfection efficiency in cells transfected with miR-NC, miR-516b-5p mimics and inhibitors. (D) hsa_circ_0072107, miR-516b-5p and miR-510-5p levels in RNA enriched by circ_0072107 probe or scramble probe during biotinylated probe pull-down assay. (E) hsa_circ_0072107 level in miRNA complexes enriched by anti-AGO or IgG during anti-AGO2 RIP assay. AC16 cells transfected with miR-516b-5p mimic or miR-NC were used. (F) The effect of miR-516b-5p mimic or miR-NC on the relative luciferase activity of luciferase reporter with linear circ_0072107 or linear mutcirc_0072107 as the artificial 3′ UTR of Renilla , respectively. *P<0.05, **P<0.01, n=3. miRNA, microRNA; AGO2, argonaute 2; RIP, RNA immunoprecipitation; NC, negative control.

    Techniques Used: Binding Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Biomarker Discovery, Transfection, Pull Down Assay, Luciferase, Activity Assay, RNA Immunoprecipitation, Negative Control



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    ez-magna rip rna-binding protein immunoprecipitation kit  (Millipore)
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    Millipore ez-magna rip rna-binding protein immunoprecipitation kit
    miR-516b-5p is a miRNA binding to hsa_circ_0072107. (A) Venn diagram showed that two miRNAs may bind to hsa_circ_0072107. (B) The binding site information of miR-516b-5p and miR-510-5p on hsa_circ_0072107. (C) Reverse transcription-quantitative PCR validation of transfection efficiency in cells transfected with miR-NC, miR-516b-5p mimics and inhibitors. (D) hsa_circ_0072107, miR-516b-5p and miR-510-5p levels in RNA enriched by circ_0072107 probe or scramble probe during biotinylated probe pull-down assay. (E) hsa_circ_0072107 level in miRNA complexes enriched by anti-AGO or IgG during anti-AGO2 <t>RIP</t> assay. AC16 cells transfected with miR-516b-5p mimic or miR-NC were used. (F) The effect of miR-516b-5p mimic or miR-NC on the relative luciferase activity of luciferase reporter with linear circ_0072107 or linear mutcirc_0072107 as the artificial 3′ UTR of Renilla , respectively. *P<0.05, **P<0.01, n=3. miRNA, microRNA; AGO2, argonaute 2; RIP, RNA <t>immunoprecipitation;</t> NC, negative control.
    Ez Magna Rip Rna Binding Protein Immunoprecipitation Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    METTL3 positively regulates MALAT1 expression. <t>A.</t> <t>RT-qPCR</t> measured MALAT1 expression in healthy controls (42 cases) and MM patients (56 cases); B. RT-qPCR detected MALAT1 expression levels in transfected cells; C. Correlation between METTL3 and MALAT1; D. <t>RIP-qPCR</t> experiment detected the interaction between METTL3 and MALAT1; E. Detection of the m6A level of MALAT1 after downregulating METTL3; F. Detection of m6A modification of MALAT1 by MeRIP-qPCR; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the si-NC group; # P < 0.05 vs. the pcDNA-NC group.
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    ez-magna rip kit  (Millipore)
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    Millipore ez-magna rip kit
    METTL3 positively regulates MALAT1 expression. <t>A.</t> <t>RT-qPCR</t> measured MALAT1 expression in healthy controls (42 cases) and MM patients (56 cases); B. RT-qPCR detected MALAT1 expression levels in transfected cells; C. Correlation between METTL3 and MALAT1; D. <t>RIP-qPCR</t> experiment detected the interaction between METTL3 and MALAT1; E. Detection of the m6A level of MALAT1 after downregulating METTL3; F. Detection of m6A modification of MALAT1 by MeRIP-qPCR; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the si-NC group; # P < 0.05 vs. the pcDNA-NC group.
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    Image Search Results


    miR-516b-5p is a miRNA binding to hsa_circ_0072107. (A) Venn diagram showed that two miRNAs may bind to hsa_circ_0072107. (B) The binding site information of miR-516b-5p and miR-510-5p on hsa_circ_0072107. (C) Reverse transcription-quantitative PCR validation of transfection efficiency in cells transfected with miR-NC, miR-516b-5p mimics and inhibitors. (D) hsa_circ_0072107, miR-516b-5p and miR-510-5p levels in RNA enriched by circ_0072107 probe or scramble probe during biotinylated probe pull-down assay. (E) hsa_circ_0072107 level in miRNA complexes enriched by anti-AGO or IgG during anti-AGO2 RIP assay. AC16 cells transfected with miR-516b-5p mimic or miR-NC were used. (F) The effect of miR-516b-5p mimic or miR-NC on the relative luciferase activity of luciferase reporter with linear circ_0072107 or linear mutcirc_0072107 as the artificial 3′ UTR of Renilla , respectively. *P<0.05, **P<0.01, n=3. miRNA, microRNA; AGO2, argonaute 2; RIP, RNA immunoprecipitation; NC, negative control.

    Journal: Molecular Medicine Reports

    Article Title: circRNA hsa_circ_0072107 aggravates myocardial hypertrophy via its function as a competitive endogenous RNA of miR-516b-5p

    doi: 10.3892/mmr.2025.13597

    Figure Lengend Snippet: miR-516b-5p is a miRNA binding to hsa_circ_0072107. (A) Venn diagram showed that two miRNAs may bind to hsa_circ_0072107. (B) The binding site information of miR-516b-5p and miR-510-5p on hsa_circ_0072107. (C) Reverse transcription-quantitative PCR validation of transfection efficiency in cells transfected with miR-NC, miR-516b-5p mimics and inhibitors. (D) hsa_circ_0072107, miR-516b-5p and miR-510-5p levels in RNA enriched by circ_0072107 probe or scramble probe during biotinylated probe pull-down assay. (E) hsa_circ_0072107 level in miRNA complexes enriched by anti-AGO or IgG during anti-AGO2 RIP assay. AC16 cells transfected with miR-516b-5p mimic or miR-NC were used. (F) The effect of miR-516b-5p mimic or miR-NC on the relative luciferase activity of luciferase reporter with linear circ_0072107 or linear mutcirc_0072107 as the artificial 3′ UTR of Renilla , respectively. *P<0.05, **P<0.01, n=3. miRNA, microRNA; AGO2, argonaute 2; RIP, RNA immunoprecipitation; NC, negative control.

    Article Snippet: After 48 h, cells were collected using the EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (cat. no. 17-701, MilliporeSigma) according to the manufacturer's instructions.

    Techniques: Binding Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Biomarker Discovery, Transfection, Pull Down Assay, Luciferase, Activity Assay, RNA Immunoprecipitation, Negative Control

    METTL3 positively regulates MALAT1 expression. A. RT-qPCR measured MALAT1 expression in healthy controls (42 cases) and MM patients (56 cases); B. RT-qPCR detected MALAT1 expression levels in transfected cells; C. Correlation between METTL3 and MALAT1; D. RIP-qPCR experiment detected the interaction between METTL3 and MALAT1; E. Detection of the m6A level of MALAT1 after downregulating METTL3; F. Detection of m6A modification of MALAT1 by MeRIP-qPCR; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the si-NC group; # P < 0.05 vs. the pcDNA-NC group.

    Journal: Journal of Bone Oncology

    Article Title: Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression

    doi: 10.1016/j.jbo.2025.100695

    Figure Lengend Snippet: METTL3 positively regulates MALAT1 expression. A. RT-qPCR measured MALAT1 expression in healthy controls (42 cases) and MM patients (56 cases); B. RT-qPCR detected MALAT1 expression levels in transfected cells; C. Correlation between METTL3 and MALAT1; D. RIP-qPCR experiment detected the interaction between METTL3 and MALAT1; E. Detection of the m6A level of MALAT1 after downregulating METTL3; F. Detection of m6A modification of MALAT1 by MeRIP-qPCR; the data were expressed as mean ± standard deviation. * P < 0.05 vs. the si-NC group; # P < 0.05 vs. the pcDNA-NC group.

    Article Snippet: RNA-RIP followed by qPCR was performed using the Magna RIP TM Kit (Millipore, USA).

    Techniques: Expressing, Quantitative RT-PCR, Transfection, Modification, Standard Deviation